Abstract
Phosphate mineral ores are non-renewable resources. It is the main source of fertilizers and phosphorous chemicals. The enrichment process is essential to meet the industrial specifications through the separation of undesirable gangues. The biological processes are more attracted to mineral processing due to their lower operating costs and easy mineral ores applications. Bacteria can bring out surface modification leading to the separation of minerals by bio-flotation. Through this study, Pseudomonas songnenensis is isolated from phosphate ore surface and identified by 16SrRNA. The zeta potential and adhesion measurements showed that the Pseudomonas songnenensis has a higher affinity to apatite than calcite, which is confirmed by FTIR measurements. A concentration of 32.7% P2O5 and 6.8% CaCO3 has been obtained from a binary mixture with 25% P2O5 and 20% CaCO3%. By applying the optimum conditions on natural phosphate ore, a concentrate with 31.5% P2O5 has been obtained from a feed containing 21.2% P2O5 in the presence of 4×107 cells/ml (Pseudomonas songnenensis) at 25C and pH 6.5.
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