Abstract

Metamorphic proteins are an emerging class of proteins that defy the classic 'one structure-one fold' paradigm by alternating between two conformations despite having the same primary structure (i.e. sequence). KaiB is the only metamorphic protein known to exist in any circadian clock. It is a component of the cyanobacterial clock, adopting one fold during the day and a distinctly different one at night. Our recent findings suggest that three out of seven prolyl residues in KaiB- P63, P70 and P72- adopt the trans configuration during the day and cis at night. Also, there is a fourth prolyl residue, P51, which even though is trans in both folds is critical to clock mechanism. Binding assays of prolyl-to-alanyl KaiB mutants suggest hierarchical roles of the prolyl residues and raise the possibility that they isomerize sequentially: P63->P70 ->P72.

Full Text
Paper version not known

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.