Abstract
A reliable isolation procedure is elaborated for the purification of ecdysteroids from Serratula wolffii. The procedure is also applicable to other plant sources. The general ecdysteroid isolation procedure was improved by the introduction of preparative rotation planar chromatography (RPC) to the purification process. Effective and simple cleanup and vacuum reversed‐phase column chromatographic separation was completed with RPC, or repeated RPC, to obtain pure ecdysteroids (ajugasterone C, dacryhainansterone, 22‐deoxy‐integristerone A, 20‐hydroxyecdysone, and 2 new ecdysteroids) from the crude extract. This paper discusses the advantages of this method as the final step of ecdysteroid isolation.
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