Abstract
RECONSTITUTION of bacterial ribosomes occurs in vitro in a system containing ribosomal components only1, which suggests that ribosomes also form by self-assembly in vivo. This inference is reinforced by the fact that intermediary particles, containing a similar fraction of ribosomal proteins, are observed both in vitro and in vivo1–3. The in vitro reconstitution process, however, requires a very high energy of activation, presumably to force the intermediates into a rare conformational state1,4. This requirement is too high to be compatible with the rate of ribosome formation in vivo5. Furthermore, there is evidence that the rate-limiting step which leads to the accumulation of the precursor particles in vivo does not involve spontaneous conformational change of these particles6. Although all the information, therefore, required to construct a ribosome must be present in its components, it seems that cells use an additional mechanism to facilitate their assembly.
Published Version
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