Abstract
Rats were injected intravenously or subcutaneously with 3H-H2O and then intravenously with serum containing 14C-labeled triglyceride (TG), collected from a donor rat previously injected with palmitate-1-14C. Recipient animals were studied at various times in relation to feeding, and thus had widely different rates of adipose tissue lipogenesis during the 30-minute observation period before sacrifice. Analyses of the time course of 14C of plasma TG, of the 3H content of the plasma TG and of liver lipid at sacrifice, and of the 14C/3H ratio in adipose tissue led to the conclusion that transport through the plasma plays an insignificant role in the deposition of newly synthesized fatty acid in the adipose tissue of the rat.
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