Abstract
This paper presents the effect of pico pulse electric field on intra organelle nano poration of multi layer osteoblast cell placed in a 3D non uniform microfloudic chip composed of bi metallic heterogeneous micro electrode under the influences of smart control FPGA based pico pulse generator and images of intra organelle nanoporation are recognized by neural fuzzy network. It is observed that When the micro pulse is applied the cell starts to response but it is unable to penetrate the intra cellular nucleus- membrane where as the expected results will come when the Pico pulse is applied on the cell, a number of nana pores are generated on the intra organelle and chemicals are entered into the cell. It is also exposed that the key parameter of nanoporation such as intra organelle voltage, pore radius, pore density, pressure, surface tension and ion uptake are externally controlled by user defined hybrid 3D micro chip and FPGA based pico pulse generator. The application of neural fuzzy network for succesfull reorganization of nanoporative image is completely explore.
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