Role of P-type ATPase metal transporters and plant immunity induced by jasmonic acid against Lead (Pb) toxicity in tomato

Abstract

The phytohormone jasmonic acid (JA) plays an imperative role in plants by modulating the activity of their antioxidative defense system under stress conditions. Here, we explored the role of JA-induced alterations in the growth and transcript levels of antioxidative enzymes in tomato seedlings exposed to different Pb concentrations (0.25, 0.50, and 0.75 mM). Pb treatment caused a dose-dependent reduction in their root and shoot lengths. Treatment of 0.75 mM Pb showed an increase in the contents of malondialdehyde (MDA), superoxide anion (O2•−), and hydrogen peroxide (H2O2) as compared to the untreated seedlings. Pb uptake was enhanced with an increase in Pb concentration. The seeds primed with JA showed reduction in Pb uptake and improvement in growth under Pb toxicity. The seedlings treated with both JA (100 nM) and Pb (0.75 mM) showed a decline in the levels of MDA, O2•−, and H2O2 as compared to the seedlings treated with 0.75 mM Pb alone. These results suggested that JA (100 nM) mitigated the oxidative damage by lowering the expression of the RBO and P-type ATPase transporter genes and by modulating antioxidative defense system activity. The biochemical and molecular analyses showed that JA plays a crucial role in plant defense responses against Pb stress.