Role of omega oxidation of fatty acids in formation of the acetyl unit for acetylation.

E Hemmelgarn,K Kumaran,B R Landau

doi:10.1016/s0021-9258(17)40275-4

Abstract

Role of omega oxidation of fatty acids in formation of the acetyl unit for acetylation.

Highlights

W-labeled fatty acids and 2-amino-4-phenylbutyric acid were administered to fed and starved normal and nonketotic and ketotic diabetic rats
We developed an approach for estimating the contribution of o oxidation to fatty acid oxidation [13]
The I-14C-labeled fatty acids yielded acetate from the acetylated 2-amino-4-phenylbutyric acid with over 98% of its i4C in carbon 1

Summary

PROCEDURES

Chemicals - m-2-Amino-4-phenylbutyric acid and its acetylated derivative were prepared according to the method of du Vigneaud and Irish [18]. Thirty-six hours after the last injection and for every 12 h thereafter their urines were tested for glucose and ketones with the use of Labstix and Ketostix, Ames Co., Elkart, Indiana Those rats which developed and maintained marked glucosuria, but not ketonuria, for 5 to 9 days had added to their diet 1 g of nn-2-amino-4-phenylbutyric acid per 100 g of the stock diet [14]. They ingested 15 to 20 g of the mixture per day They were treated by injection with the labeled fatty acids, their urines collected, and killed in the same manner as the nonketotic diabetic rats. Another group of normal rats was fasted. It provides a measure of the purity of the sample and the adequacy of the degradation

RESULTS

TABLE I

TABLE IV

DISCUSSION