Abstract
The present study aimed to determine whether oleanolic acid (Ole) could be used to treat psoriasis and its related underlying mechanism of action via in vitro analysis. HaCaT cells were stimulated with IL-22 to established an in vitro psoriasis cell model. MTT, flow cytometry and TUNEL assays, respectively. Transmission electron microscopy was used to observe the cell ultrastructure. LC3B protein expression levels were analyzed using immunofluorescence, other protein expression levels were determined using western blotting. Cell viability was significantly increased, while the apoptotic rate was significantly decreased in the model group (P < 0.001). In addition, Notch1, Hes1, beclin 1 and LC3B protein expression levels were significantly downregulated, while P62 protein expression levels were significantly upregulated in the model group compared with the control group (P < 0.001). Supplementation of Ole, the increased levels of proliferation were significantly suppressed, while cell apoptosis was significantly increased (P < 0.05) in a dose-dependent manner, which was discovered to occur via Notch1 upregulation. Notably, the transfection with small interfering RNA-Notch1 significantly reversed the effect of Ole treatment (P < 0.001) and the levels of autophagy were also decreased. In conclusion, the findings of the current study suggested that Ole may relieve psoriasis via upregulating Notch1, which subsequently regulates cell autophagy.
Highlights
Psoriasis is a common and recurring chronic inflammatory skin condition, and its prevalence among adults ranges from 0.91 to 8.5% in different Please clarify, are you referring to different countries and ethnic (Griffiths et al 2017)
After oleanolic acid (Ole) intervention, compared with the model group, the proliferative rates in the Ole groups were significantly inhibited, and significant differences were identified in the proliferative rates among the different Ole groups. 25.0, 50.0 and 100.0 μmol/L Ole was safe to HaCaT cell in normal treatment; in HaCaT cell psoriasis model, 25.0, 50.0 and 100.0 μmol/L Ole had effects to depress HaCaT hyperproliferation
The results of the present study revealed that Ole intervention could effectively inhibit the abnormal proliferation of HaCaT cells induced by IL-22 stimulation and promote their apoptosis, which may subsequently inhibit the development of psoriasis
Summary
Psoriasis is a common and recurring chronic inflammatory skin condition, and its prevalence among adults ranges from 0.91 to 8.5% in different Please clarify, are you referring to different countries and ethnic (Griffiths et al 2017). The exact pathogenesis of psoriasis remains unclear. Studies have confirmed that the onset of psoriasis is closely associated with autoimmunity and inflammation, and the excessive proliferation of keratinocytes and parakeratosis are key factors underlying its pathogenesis (Perera et al 2012). It was previously reported that oleanolic acid (Ole), which is an extensively used plant-derived triterpenoid, exerted effective antioxidant effects in cell model (Guo et al 2020). Ole was discovered to exert therapeutic benefits in liver cancer (Zhou et al 2020), diabetes-induced cell injury (Chen et al 2019) and bladder cancer (Song et al 2017) via regulation of autophagy.
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