Role of oestrogen and its receptors in HEV-associated feto-maternal outcomes.

Abstract

Pregnant women infected with HEV develops adverse pregnancy outcomes like, abortions, intrauterine fetal death, still births, neonatal deaths, preterm delivery and maternal mortality. To correlate oestrogen and its receptors ESR1α and ESR2β levels with HEV-associated feto-maternal outcomes. A total of 142 pregnant women with HEV infection and 142 pregnant controls were included in study from Department of Obstetrics & Gynaecology and Department of Medicine, Maulana Azad Medical College (MAMC) and associated Lok Nayak Hospital (LNH), New Delhi. Three millilitre of blood sample was collected in plain for quantification of oestrogen, and its receptors ESR1α and ESR2β using commercially available third-generation ELISA kits. The levels of oestrogen, ESR1α and ESR2β were considerably higher in HEV-infected pregnant women (20.11±18.19ng/mL, 10.58±3.27ng/mL, 10.42±4.71ng/mL respectively) than pregnant controls (11.74±6.42ng/mL, 9.11±1.63ng/mL, 9.01±1.18ng/mL respectively)(P<0.0001). It was found that oestrogen levels were significantly higher in pregnant women infected with HEV who had preterm delivery, low birth weight babies and fetal loss (19.64±17.60ng/mL, 19.71±17.63ng/mL, 33.62±23.20ng/mL respectively) than who had full term delivery, average birth weight babies and live babies (11.71±8.77ng/mL, 11.99±9.44ng/mL, 16.58±14.98ng/mL respectively)(P<0.05). A significant negative correlation was observed between baby birth weight and oestrogen levels in HEV-infected pregnant women. The high level of oestrogen plays an important role in preterm delivery, low birth weight babies and fetal mortality in pregnant women with HEV infection through placental dysfunction. Moreover, oestrogen level is a significant predictor for preterm delivery and maternal mortality and ESR2β levels is a significant predictor for maternal mortality in pregnant women infected with HEV.