Role of nuclear factor-κB in macrophage inflammatory protein-1α expression in lung of rats with ventilator induced acute lung injury

Abstract

Objective To approach the regulating role of nuclear factor KB (NF-κB) in macrophage inflammatory protein-1α (MIP-1α) expression in lung of rats with ventilator induced acute lung injury by observing the expressions of NF-κB p65 protein and MIP-1α mRNA in lung of rats with different tidal volumes of ventilation. Methods Twenty-four health male Wistar rats were randomly divided into control group,low tidal volume group and high tidal volume group. The expressions of MIP-1α mRNA and NF-κB p65 protein in lung tissue of rats were detected with hybridization in situ and immunohistochemistry respectively. Results The positive percentages of expressions of NF-κB p65 protein and MIP-1α mRNA in bronchiole epithelial cells of rats in high tidal volume group were significantly higher than those in low tidal volume group and control group (all P <0. 01). There was no statistical significance between low tidal volume group and control group. Correlation analysis showed that the positive percentage of NF-κB p65 protein expression was positively correlated with that of MIP-1α mRNA expression in bronchiole epithelial cells of all groups ( r =0. 482, P <0. 05). Conclusions The high-expression of MIP-1α mRNA in lung tissue initiated by high tidal volume ventilation has certain effect on ventilator induced lung injury, and to some extent,the expression of MIP-1α in lung tissues may be regulated by NF-κB. Key words: Mechanical ventilation; Acute lung injury; Nuclear factor-κB; Macrophage inflammatory protein-1α