Role of nod-like receptor family pyrin domain containing 3 and interleukin-1β on type 2 diabetes mellitus with chronic periodontitis

Renfei Zhang ,Hong Yang

doi:10.3760/cma.j.issn.1674-5809.2017.08.009

Abstract

Objective Nod-like receptor family pyrin domain containing 3 (NLRP3) and interleukin-1β (IL-1β) were detected for exploring the interactive mechanism between type 2 diabetes mellitus (T2DM) and chronic periodontitis (CP). Methods Hospitalized T2DM patients,aged 20-80 years old were divided into T2DM+CP group (n=70), T2DM group (n=40), CP group (n=30) and healthy control group (n=35). The concentration of IL-1β protein were tested by ELISA assays and the expression of NLRP3 mRNA were detected by RT-PCR. The differences of the concentrations of IL-1β and the levels of the NLRP3 inflammasomemRNA were compared by ANOVA (analysis of variance) ,the relationships between 4 groups were evaluated by pearson test or spearman test. Results The up-regulation of serum IL-1β concentrations was detected in the T2DM+CP group compared with the healthy group, the CP group and the T2DM group[ (41±10) , (28±6) , (34±8) , (31±7) ng/L, F=23.092, P<0.05]. The expression of NLRP3 mRNA of the T2DM+CP group was higher compared with the healthy group, the CP group and the T2DM group (1.9±0.5, 1.0, 1.4±0.6, 1.4±0.5, F=33.076, P<0.05) . The expression of ASCmRNA of the CP group, the T2DM group and the T2DM+CP group was higher compared with the healthy group (3.0±1.2, 2.9±1.9, 2.7±0.9, 1.0, F=23.756, P<0.05) . The expression of caspase-1 mRNA of the CP group and the T2DM+CP group was higher compared with the healthy group (1.5±0.4, 1.4±0.6, 1.0, F=19.155, P<0.05) .The expression of NLRP3 mRNA and caspase-1 mRNA were correlated positively with the serum IL-1β concentrations (r=0.343, 0.227, all P<0.05) . Conclusion The increased serum IL-1β concentration and NLRP3 mRNA expression may promote the chronic inflammatory response and aggravate the progression of the disease in patients with T2DM and CP. Key words: Diabetes mellitus, type 2; Chronic periodontitis; Interleukin-1β; Nod-like receptor family pyrin domain containing 3

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