Abstract

AbstractCapability of strains of Rhizobium and Bradyrhizobium to solubilize phosphate was determined in a defined medium containing hydroxyapatite as source of insoluble phosphate and (NH4)2SO4 or KNO3 as nitrogen source. Phosphate solubilization was found to be directly related to reduction of medium pH during growth of the strains. In general, presence of NH in the medium caused higher solubilization of phosphate by the strains as compared to the presence of NO as nitrogen source. The strain R. leguminosarum bv. viccae BICC 635 was the only exception which solubilized comparable amount of phosphate in medium containing either (NH4)2SO4 or KNO3. It appears, that the strains employ two different mechanisms for phosphate solubilization. One of the mechanisms relies upon the presence of NH in the culture and the other is independent of it. The strains could be divided into two groups, high‐ and low solubilizers based on their performances of phosphate solubilization.

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