Abstract
CLONAL nerve and muscle cells interact in vitro to produce a nerve-muscle contact which is associated with an area of increased acetylcholine sensitivity on the muscle1,2. This association is reminiscent of the distribution of acetylcholine sensitivity on innervated skeletal muscle in vivo3,4. It is possible to use cultures of nerve and muscle cells to examine the requirements for localization in vitro. A previous report showed that cholinergic transmission is not required2. Here I discuss the requirement for muscle electrical and contractile activity in localisation of acetylcholine sensitivity.
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