Role of MTHFR C667T and MTRR A66G genes polymorphism with thyroid disorders

Abstract

Thyroid disorders is the most common disease of the endocrine system. It is estimated that there are approximately 300,000 new cases of thyroid cancer worldwide. Overcome of these are females, the number of cases for thyroid disorders has been infix increasing. The aim of this study was to determine the correlation between MTHFR C677T and MTR A2756G polymorphisms involved in folic acid metabolism with thyroid disorders. We also wanted to investigate the relationship between these genetic variation and risk factors. The blood samples were collected from all cases in two different tubes: first for biochemical analysis, the second have EDTA used for DNA extraction. By Bio drop, DNA concentration and purity were measuring done, In this study be accomplished the correlation between the genetic variation of MTRR A66G gene and MTHRF C677T gene polymorphism with thyroid disorders by ARMS-PCR. In this study show increase the levels of T3 and T4 and decrease the level of TSH in patients with hyperthyroidism compare with control groups, also this table show decrease the levels of T3 and T4 but increase the level of TSH in patients with hypothyroidism compare with healthy peoples. As well as the result showed increase in levels of glucose, cholesterol and triglyceride in patients with hypothyroidism compare with healthy people, Concerning in hyperthyroidism we showed decrease in levels of glucose, cholesterol and triglyceride compare with healthy people. The association between MTHFR C677T and MTRR A66G polymorphisms and the risk of thyroid disorders showed. The 677TT genotype was associated with increased risk for both hypothyroidism (30 %) and hyperthyroidism (10 %) but in different ratio. The 677TC genotype be found in this study have same influence in hypothyroidism (60 %) and hyperthyroidism (60 %) and the 677CC genotype present in result but have different effect on study groups in hypothyroidism (10 %) and hyperthyroidism (30 %). The result also show the genotype ratio of MTRR A66G gene in cases. The GG genotype was associated with thyroid disorders for both hypothyroidism (60 %) and hyperthyroidism (40 %) but in different ratio. The AG genotype be found in this study have same influence in hypothyroidism (20 %) and hyperthyroidism (20 %) and the AA genotype present in result but have different effect on study groups in hypothyroidism (20 %) and hyperthyroidism (40 %). The study has found that the correlation and interaction between MTHFR and MTRR genes polymorphism with thyroid disorders. In addition, we found the genotype (TT) MTHFR C677T polymorphism and the genotype (GG) for MTRR A66G increased risk factors in thyroid disorders.