Role of microRNA-22 and microRNA-1825 in the diagnosis and differential diagnosis of juvenile systemic lupus erythematous

Abstract

Objective To explore the role of microRNA(miR)-22 and miR-1825 in the diagnosis and differential diagnosis of juvenile systemic lupus erythematous(JSLE). Methods The cases of JSLE hospitalized in Capital Institute of Pediatrics Teaching Hospital Affiliated to Peking University from June 2013 to May 2014 were selected as study group.The cases with systemic juvenile idiopathic arthritis(sJIA), nephrotic syndrome(NS), Kawasaki disease(KD), Henoch-Schonlein purpura(HSP)were selected as patients control group.The healthy children were selected as healthy control group.The expression levels of miR-22 and miR-1825 in the plasma of JSLE, sJIA, NS, KD, HSP and healthy children were detected by using real-time PCR respectively.Receiver operating characteristic curve (ROC) analysis was performed to evaluate the value of miR-22 and miR-1825 miRNA as a biomarker with the sensitivity and specificity.Three data bases, included Targetscan, PicTar and miRanda, were applied to predict the target gene.The target gene was analyzed by adopting Gene Ontology (GO) in terms of molecular function, biological process and cellular component, and by adopting Kyoto Encyclopedia of Genes and Genomes(KEGG) in terms of pathway. Results Compared with healthy children, the amount of miR-22 and miR-1825 in JSLE patients were lower, and there were significant differences(t=-3.076, -9.054, P 0.05). The area under ROC curve(AUC) of miR-22 between JSLE and healthy children was 0.777.The AUC of miR-1825 between JSLE and healthy children was 1.000.The AUCs between JSLE and controls of sJIA, NS, KD, HSP of miR-22 were 0.731-1.000.The AUCs between JSLE and controls of sJIA, NS, KD, HSP of miR-1825 were 0.939-1.000.There was positive relation between the amount of miR-22 and complement C3 in plasma(r=0.493, P=0.027). Conclusions The amount of miR-22 and miR-1825 in the plasma of JSLE embrace the potential of distinguishing JSLE from healthy children, sJIA, NS, KD, HSP.MiR-22 has the ability to predict the activity of JSLE. Key words: Juvenile systemic lupus erythematous; MicroRNA-22; MicroRNA-1825; Diagnosis; Differential diagnosis