Abstract
Adequate individual supplements of Zn, Co and Mo increased chemical oxygen demand (COD) removal and specific oxygen uptake rates during activated sludge treatment of dyeing wastewater, while overdoses of micronutrients can decrease metabolic rates. The effects of combing Zn, Co, and Mo at different doses were investigated using response surface methodology, with a second order polynomial equation: Y = 78.19+ 0.17X1 + 37.33 X2 + 20.20X3 - 5.58X1X2 - 63.05 X2X3, where Y means COD removal rate (%), X1 means Co concentration, X2 means Zn concentration, X3 means Mo concentration. The maximum COD removal efficiency of 89.41% was achieved with doses of 0.02 mg/L Co, 0.24 mg/L Zn and 0.45 mg/L Mo. Both the combinations of Zn-Co and Zn-Mo acted antagonistically for COD removal efficiency, and the combination of Co-Mo was negligible to COD removal efficiency. Doses of micronutrients, particularly Zn, Co and Mo, were beneficial for microbial shift towards Planctomyces and Bacteroidetes.
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