Abstract
Fusarium oxysporum β-glucosidase catalyses the transglycosylation and reverse hydrolysis reaction in an aqueous solution, in the presence of a second nucleophile stronger than water, such as methanol. In transglycosylation, the K m values of cellobiose for the trans reaction and hydrolysis were 138.2mM and 356.5mM respectively and the competitive role of methanol was shown. The K m value of methanol was calculated to be 1.41M. In the reverse hydrolysis the influence of the donor concentration, up to 90% w/v glucose, was investigated and the competitive role of methanol as a nucleophilic acceptor of the glycosyl moiety was also demonstrated.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
