Abstract
The studies on the decay of the pH difference, ΔpH, across soyabean phospholipid vesicular membrane have shown that the rates of net proton transport and the associated Li + and Na + ion transport across the membrane can be enhanced by the combined action of gramicidin, valinomycin and carbonyl cyanide m-chlorophenylhydrazone (CCCP) in K +-free vesicle solutions. The data obtained under different experimental conditions suggest that this enhancement is a consequence of facilitation of CCCP − transport (1) by complexing CCCP − with the highly membrane permeant valinomycin without the metal ion bound to it and (2) by the associated Li + or Na + transport through the gramicidin channel such that no net charge is transported across the membrane. The dissociation constant of the weak valinomycin-CCCP − complex has been estimated to be >200 mM in the membrane. The ΔpH in these experiments were created by temperature jump.
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