Role of Manganese in Calcium Metabolism at the Level of the Enteral and Internal Environment of the Body

Abstract

The authors studied the effect of manganese on calcium metabolism at the level of the enteral and internal environment of the body in an experiment on rabbits. Two groups (control and experimental) of rabbits selected by the paired-analysis method were used in the experiment. There were three animals in each group. The animals in the experimental group were given a diet containing three times the amount of manganese. At the end of the experiment, blood was collected from various parts of the digestive tract of all animals under general anaesthesia. Organs and tissues werecollected for analysis after control slaughter, and the intestines were removed, followed by sampling of chyme and mucosa from the walls of various sections. The chyme was separated into fractions using the developed method. The concentration of manganese and calcium in intestinal chyme and its fractions, in mucosa of the intestinal wall, in the blood flowing in and out of the digestive tract, in organs and tissues was determined by the atomic absorption spectrophotometry method. It was shown experimentally that when the concentration of manganese in the diet of rabbits was tripled, the element was not accumulated in the internal environment but was accumulated by endogenous structures of the chyme and the mucous membrane of the wall of the small and large intestines. A metabolic change in calcium metabolism was revealed when manganese was tripled in the diet: its concentrations in the blood flowing from the intestines and in the mucous layer of the small intestine wall decreased. As a result, there was a significant decrease in bone concentrations and a downward trend in liver and kidney concentrations. The antagonistic effect of manganese on calcium metabolism in the enteric and internal environment is concluded. In the interaction of elements, the leading role of endogenous structures of chyme is noted due to ion-exchange properties of glycoproteins of intestinal cavity mucosa.