Abstract
Aims: This paper investigates the presence of M3 muscarinic acetylcholine receptor autoantibody present in the serum of patients with primary SjÖgren syndrome (pSS). Main methods: We detected the levels of M3mAChR peptide IgG, PGE2, IL-1β in serum of SS patients using the enzyme-linked immune sorbent assay (ELISA). To measure the quantity of nitrite/nitrate, we used Griess reagent system. Key findings: Titres of M3mAChR antibody in sera from SS patients are significantly enhanced compared to healthy subjects (control). The enhancement of these autoantibodies is accompanied by the increase of the levels of PGE2, IL-1β and nitrite/nitrate in serum. Under in vitro conditions, the synthetic human M3 peptide impaires the increment of M3mAChR antibody but not that of nati-Ro/SSA antibody. In positive anti-Ro/SSA antibody patients, the increment of M3mAChR peptide IgG and the measured pro-inflammatory substances is related. Significance: On this basis, anti M3mAChR peptide IgG can be said to act as a modulator of the immune system and to play a role in the host-chronic increment of proinflammatory substances in SS patients with positive Ro/SSA antibody. This association between the antibody and the pathogenesis of SS disease may result in useful predicting SS.
Highlights
Sjögren’s syndrome (SS) is a systemic autoimmune disease primarily affecting the exocrine glands
To demonstrate the presence of serum IgG directed against M3mAChR antibodies, we performed an enzyme-linked immune sorbent assay (ELISA) assay
The coating antigen used for this purpose was a M3 human synthetic peptide corresponding to the amino acid sequence of the second extracellular loop (K-R-T-V-P-D-N-Q-C-F-I-Q-F-L-S-N-P-A-V-TF-G-T-A-I) of human glandular M3mAChR
Summary
Sjögren’s syndrome (SS) is a systemic autoimmune disease primarily affecting the exocrine glands. The disease occurs either in a primary form (pSS) or in association with other autoimmune diseases (associated form, aSS) such as systemic lupus erythematosus (SLE), rheumatoid arthritis (RA) and systemic sclerosis (SSc). The presence of serum autoantibodies in the course of SS is of key significance. They are usually used as biomarkers for the classification and diagnosis of pSS and aSS [4] [5]. Other autoantibodies not currently used in clinical practice such as anti-M3 muscarinic acetylcholine receptor (anti-M3mAChR), have been described in pSS/aSS [6] [7] [8] as useful markers [9] [10]
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