Abstract
Biofilm development on the membrane surface is one of the main reasons for membrane fouling in membrane bioreactors (MBRs) and it is a big problem for their stable operation. Precise information on the microbial community composition of the biofilm is needed for a better understanding of biofilm development. However, there have been limited investigations of the relationship between the biofilm formation process and the microbial community of activated sludge and biofilm in MBRs treating real sewage. In this study, relationships between the microbial community structure of biofilm and activated sludge at each biofilm formation stage were investigated and biofilm growth was elucidated by nondestructive observations. Two anoxic/oxic MBRs were operated and membrane fouling was induced. Permeability rapidly decreased in both reactors and live cell microcolonies were formed on dead cell conditioning film on the membrane surface. Principal component analysis based on 16S rRNA gene sequences showed that the biofilm microbial community changed significantly from middle stage to mature biofilm when compared with that of activated sludge. The abundance of specific bacteria, such as unclassified Neisseriaceae, increased in middle-stage biofilm and the diversity indexes of middle-stage biofilm were lower than those of mature biofilm and activated sludge. These results suggested that the presence of specific bacteria with colonization ability played a crucial role in biofilm formation. Strategies are needed to target membrane fouling mitigation during early- and middle-stage biofilm formation to reduce MBR membrane fouling. KEY POINTS: • Microbial community of mature biofilm was approached to that of activated sludge. • In the middle-stage biofilm, live cells colonized on a dead-cell-conditioning-film. • Microbial diversity was lower in live cell colonizing stage than in activated sludge.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
