Abstract
The presence in the system of critical liquid-liquid phase transition (PT) by the mechanism, resulting in formation of dispersion system, namely high-concentrated emulsion, has been established here during cooling when using polarized light microscopy and fixation of critical opalescence phenomenon in erythrocyte concentrate with glycerol-containing cryopreservative agent, designed at the Central Research Institute of Haematology and Blood Transfusion (Russia) (CRIHBT-115 ). The studied cryobiological system displayed no signs of crystallization. A phase behaviour of cryopreservative and supernatant has been studied during cooling-warming cycle. Changes in the volume of cryopreservative and erythrocyte concentrate were comparatively and qualitatively evaluated during cooling. The mechanism of protective action of cryopreservation solution has been determined. The similarity between physical and chemical processes during cooling-warming of erythrocyte cytoplasm and garlic meristem cells (germinal plant tissue) when entering cold anabiosis has been established.
Highlights
Ключові слова: фазові перетворення типу рідина-рідина, кристалізація, склування, дисперсні системи, кріоконсервування, кріоушкодження, кріопротектори, еритроцити, поляризаційна мікроскопія
Кінце- The own investigations showed the hemolysis вий результат процесу охолодження-нагрівання during donor erythrocyte concentrate cooling with клітинної зависі, який виражається в збереженні no cryopreservative supplement to result from the цілісності клітин тільки за присутності в системі liquid-liquid phase transition (LLPT), since no liquidcrystal phase transition (LCPT) was recorded during such кріоконсерванта і залежить від кількісних харак- a system cooling [22]
The glass tubes filled with CRIHBT-115 cryopreservative were not destroyed during cooling (Fig. 6B). These results suggest an increased volume of erythrocyte cytoplasm during cooling as a result of LLPT implementation to be the main cause of their hemolysis
Summary
Ключові слова: фазові перетворення типу рідина-рідина, кристалізація, склування, дисперсні системи, кріоконсервування, кріоушкодження, кріопротектори, еритроцити, поляризаційна мікроскопія. In the erythrocyte concentrate, processed with cryopreservative agent and cooled under similar conditions, the dark zones specific for critical opalescence were formed during PT [2, 15]
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