Role of liposomes in selective proliferation of splenic lymphocytes.

Abstract

The effects of free and encapsulated allergens of Artemisia scoparia pollen on lymphocyte proliferation and immunoglobulin production in BALB/c mice were investigated. Splenic lymphocytes from mice immunized with liposome entrapped allergen (LEA) elicited a marked proliferative response upon in vitro stimulation with both free and encapsulated allergen in comparison to mice immunized with free allergen (FA). The serum immunoglobulin profile of mice administered LEA revealed a predominance of IgG1 antibodies concomitant with an enhancement of IgG2a, IgG2b, IgG3 and IgM responses and suppression of IgE responses. However immunization with FA resulted in significant production of IgE responses and low levels of IgG antibodies. The differential ability of free and encapsulated allergens to selectively induce immunoglobulin isotypes suggests that different presentation and T cell differentiation pathways may be followed by FA and LEA in the immune system. Proliferation studies involving macrophage depletion demonstrated that macrophages play an obligatory role in the processing of LEA. Analysis of cytokine production in sera of immunized mice (FA/LEA) revealed that LEA induced significant IFN-gamma responses and lower IL-4 responses than mice immunized with FA. The results of the present study indicate that liposomes synergise the proliferation by the antigen incorporated in it and polarizes the response towards Th1 type of cytokine production. The immunoadjuvant and immunomodulation property of liposomes make it an efficient vehicle for effective immunotherapy.