Role of interleukin-6 in a glucan-induced model of granulomatous vasculitis

Abstract

The role of interleukin-6 (IL-6) in granulomatous vasculitis is not well understood. To investigate its involvement in this type of vasculitis a model of glucan-induced pulmonary vasculitis employed interleukin-6 deficient (IL-6 −/−) mice. Briefly, IL-6 −/− mice and C57B/J6 wild type (IL-6 +/+) mice were injected intravenously with a suspension of glucan isolated from the cell wall of bakers yeast which results in a granulomatous vasculitis primarily in the pulmonary vasculature. Histological examination demonstrated no significant difference in the number of infiltrating leukocytes between the IL-6 +/+ and IL-6 −/− glucan-injured mice. Similar numbers of granulomas were noted in both the IL-6 +/+ and IL-6 −/− injured animals, while no granulomas were seen in saline injected control mice. Cells recovered from the bronchoalveolar lavage (BAL) fluid were differentially stained and counted. While there was a significant increase in infiltrating leukocytes recovered from the BAL following glucan-induced injury, there was no significant difference between the IL-6 +/+ and IL-6 −/− mice. In addition, no difference was demonstrated in total protein content in the BAL fluid between IL-6 +/+ and IL-6 −/− mice. However, myeloperoxidase (MPO) activity in the lungs of the IL-6 −/− mice was less than in their IL-6 +/+ counterparts suggesting that these animals have a partial defect in their ability to recruit neutrophils in this model. Studies done to look for levels of other cytokines/chemokines in these animals to compensate for the loss of IL-6 revealed that only IL-10 in the sera ( p < 0.016) and BAL fluid ( p < 0.05) of IL-6 −/− mice was significantly higher then their IL-6 +/+-injured counterparts. These studies suggest that IL-6, while possibly involved in early neutrophil accumulation in this model does not appear critical to the development of the TH-2 mediated granulomatous vasculitis.