Abstract
ObjectiveTo study the role of insulin-like growth factor II receptor in free silica-induced transdifferentiation of primary rat lung fibroblasts MethodsRat lung fibroblasts and rat alveolar macrophages were cultured. A transdifferentiation model of primary rat lung fibroblasts was induced by free silica. Levels of α-SMA protein, IGF-IIR protein and mRNA were measured by immunocytochemistry, Western blot and RT-PCR, respectively. Lung fibroblasts were treated with Wortmannin. ResultsThe expression levels of α-SMA and IGF-IIR increased with the increasing free silica concentration and decreased after Wortmannin was used. ConclusionThe IGF-IIR plays an important role in free silica-induced transdifferentiation of primary rat lung fibroblasts.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
