Abstract
Methods: Raw 264.7 cells, a murine macrophage cell line, were used in this experiment. Western blotting using IκB Ab and phosphor-specific IκB Ab was performed to evaluate the degradation and phosphorylation of IκB cells. For the IκB Kinase (IKK) activity, an immune complex kinase assay was performed. The level of interleukin-6 (IL-6) was measured by ELISA to determine the level of proinflammatory cytokine. Results: IκBα degradation began 30 min after lipopolysaccharide (LPS) treatment. However, an insulin pretreatment suppressed the IκBα degradation caused by the LPS treatment. The phosphorylation of IκBα and IKK activity was also inhibited by the insulin pretreatment. Finally, the insulin pretreatment showed a tendency to suppress the induction of IL-6 by LPS. Conclusion: Insulin might have an anti-inflammatory effect though partial inhibition of the IκB/NFκB pathway in macrophage cell lines.
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