Abstract
Yeast Saccharomyces cerevisiae is one of the simplest eukaryotic model organisms to study various parameters of ethanol tolerance and has attracted a huge consideration for the cost-conscious production of bioethanol by the fermentation of soluble sugars. Ethanol tolerance is crucial for the efficient production of bioethanol from S. cerevisiae. In this study, we examined the role of HAL5 protein kinase in ethanol tolerance in S. cerevisiae by comparing the cell growth, survival, and stress responses of wild-type, hal4,5 mutant, and Hal5p overexpressed strains under 10 % (V/V) ethanol stress. Our findings suggest that overexpression of HAL5 protein kinase enhances ethanol tolerance in S. cerevisiae by improving cellular responses to ethanol-induced stress, including trehalose accumulation, reactive oxygen species (ROS) management, glutathione homeostasis, lipid peroxidation, and protein carbonyl contents.
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