Role of Green Tea Extract in Reducing Pathological Changes of Induced Diabetes Mellitus in Rats

Kamal A Khidir,Ismail S Kakey,Sarmad R Kareem

doi:10.33899/rjs.2013.77808

Abstract

Thirty male albino rats were used to investigate the effect of green tea extract on pancreas, renal and hepatic histological parameters in alloxan induced diabetes mellitus. The animals were randomly distributed into five groups of six animals in each group. The first group was regarded as normal healthy control, the second group was regarded as diabetic control, the third group was treated with insulin, the fourth group was treated with green tea extract, 50 mg/kg body weight (GTE50), and the fifth group was treated with GTE100. This study showed that functionally diabetes related organs such as pancreas, kidney and liver showed diabetes related pathological changes and these revealed a noticeable tendency for reversion to normal after treatment with green tea extract.

Highlights

Diabetes mellitus (DM) is an increasingly common, potentially devastating, expensive, treatable, but incurable lifelong disease
The aim of this study is to investigate the effectiveness of the green tea extract in the reduction of pathological changes of alloxan induced diabetes on rat pancreas, liver and kidney
Wolfram et al, (2006) demonstrated that green tea epigallocatechin gallate possesses pronounced anti-diabetic efficacy in preclinical models of type 2 diabetes mellitus, which is at least partially mediated through reducing hepatic glucose production and enhancing the pancreatic function

Summary

Introduction

Diabetes mellitus (DM) is an increasingly common, potentially devastating, expensive, treatable, but incurable lifelong disease. DM is associated with a number of chronic complications including nephropathy, neuropathy, retinopathy, and cardiovascular disorders (Mahdi et al, 2003). Many hypoglycemic agents, such as the biguanides and sulfonylureas, are used alone or together with insulin to treat this disease, these medications can cause serious side effects, motivating a search for safer, more efficacious agents to control diabetes (Huang et al, 2005). Green tea catechins are hypothesized to help protect cells against damaging effects of reactive oxygen species by contributing, along with antioxidant vitamins C and E and enzymes superoxide dismutase and catalase, to the total antioxidant defense system (Abdel-Raheim et al, 2009). Hyperglycemia can lead to an elevated reactive oxygen species (ROS) production by respiratory enzyme chains in mitochondria (Nishikawa and Araki, 2007), formation of advanced glycation end-products (AGEs) (Monnier, 2003), and imbalance of glutathione redox status (Maritim et al, 2003)

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