Abstract
The present study demonstrated the simple, cheap, eco-friendly synthesis of the silver nanoparticles (S-AgNPs) using Symphytum officinale leaf extract. The biosynthesized S-AgNPs were characterized by UV–Vis, FE-TEM, elemental mapping, EDX, zeta potential, XRD, SAED, and FT-IR. The characterization results revealed the irregular shape and relatively stable nature of synthesized S-AgNPs. The average particle size was determined to be 87.46 nm. The zeta potential shows the negative surface charge (− 25.5 mV) of S-AgNPs. After characterization, we investigated the anti-aging effect of S-AgNPs in HaCaT keratinocyte cells. HaCaT keratinocyte cells were treated with S-AgNPs at concentrations 1, 10, 100 μg mL−1 after UVB or non-UVB irradiation. The S-AgNPs significantly inhibited the production of matrix metalloproteinase-1 and IL-6 but increased the expression of procollagen type 1. The data suggest that S-AgNPs have photoprotective properties and may have potential to be used as an agent against photoaging.Graphical abstract
Highlights
Metal nanoparticles exhibit unique chemical and physical properties including large surface/volume ratio, which is useful in different fields such as electronics, photonics, biomedical, catalysis, etc [1]
The color of S. officinale extract was changed from pale yellow to light brown in 10 min, which resembles the synthesis of AgNPs, as the particles cause surface plasmon resonance (SPR) due to which light brown color appears in the supernatant
In the UV–Vis absorption spectrum, a strong peak appeared at 468 nm for AgNPs synthesized from S. officinale (S-AgNPs), which is due to the SPR band of AgNPs (Fig. 1a)
Summary
Metal nanoparticles exhibit unique chemical and physical properties including large surface/volume ratio, which is useful in different fields such as electronics, photonics, biomedical, catalysis, etc [1]. Many standard approaches by means of physical and chemical have been used for the preparation of silver nanoparticles (AgNPs) by several researchers. The green synthesis approach provides most advantages over the chemical and physical method as it is fast, cost-effective, eco-friendly and easy to scale up for large-scale synthesis without applying high energy, high pressure, high temperature and toxic chemicals [6]. Many researchers have employed different plant parts such as root, stem, bark, leaf, fruit, bud, and latex for the synthesis of AgNPs [7,8,9,10]. Reports have suggested that nanoparticles synthesized from medicinal plants have been found to be pharmacologically active and stable, but no hazardous by-products or toxic chemicals are used in their synthesis [11]. As a green approach for the first time, we report the use of Symphytum officinale (Comfrey) leaf extract for the synthesis of AgNPs and further investigating their role in protection against UVBinduced photoaging
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