Role of Glyoxalase System on Methylglyoxal Induced Peritoneal Thickeness in Glyoxalase Transgenic Rats

Abstract

Methylglyoxal (MG) has been shown to involve in the peritoneal fibrosis in peritoneal dialysis, which is metabolized by the glyoxalase system. The present study was designed to determine the role of glyoxalase system on peritoneal thickness induced by MG.MG was intraperitoneally administered daily to either rats overexpress human glyoxalase 1 (GLO1‐Tg rats) or control rats for two‐weeks. Saline was administered as sham group. Peritoneal equilibration test was performed at the end of the study, and thickness of peritoneum was determined.Administration of MG did not change systolic blood pressure measured by tail‐cuff method in either GLO1‐Tg rats or control rats. Significant reduction in peritoneal glucose transport was observed in MG treated control rats but was not recovered in GLO1‐Tg rats. D4/D0 glucose was reduced by MG treatment but was not altered in GLO1‐Tg rats. D/P urea did not change by MG treatment. MG increased peritoneal thickness in control rats which was attenuated in those of GLO1‐Tg rats.We conclude that MG induces peritoneal dysfunction associated with peritoneal thickness. Although enhanced glyoxalase system did not recover peritoneal dysfunction, these results indicate that glyoxalase is responsible for peritoneal thickness, which could be a target mechanism for peritoneal fibrosis in peritoneal dialysis. (Support: KAKENHI23659438)