Role of glycine N‐methyltransferase in experimental ulcerative colitis (902.13)

Abstract

Inflammatory bowel diseases (IBDs) are chronic inflammatory disorders with unclear etiology and mechanism(s). Glycine N‐methyltransferase (GNMT) plays a central role in inflammatory diseases such as hepatitis and atherosclerosis. However, little is known about the impact of GNMT and the involved mechanism in the pathogenesis of IBD. In current study, we investigated the role of GNMT in colitis with the mouse model of dextran sulfate sodium (DSS)‐induced colitis. GNMT was expressed in the epithelium of the colon under normal conditions, and with DSS treatment, its expression was predominant in infiltrated leukocytes of lesions. Mice with genetic deletion of GNMT (GNMT‐/‐) showed increased susceptibility to DSS induction of colitis, as revealed by the progression of colitis. Additionally, severe colonic inflammation, including increased crypt loss, leukocyte infiltration and hemorrhage, was greater with DSS treatment in GNMT‐/‐ than wild‐type mice. Furthermore, the expression of adhesion molecule and inflammatory mediators in the colon was significantly higher with DSS treatment in GNMT‐/‐ than wild‐type mice. Moreover, loss of GNMT decreased cell apoptosis in colitis lesions with DSS treatment. Collectively, our findings suggest that GNMT may be a crucial molecule in the pathogenesis of DSS‐induced colitis. This finding may provide new information for a potential therapeutic target in treating IBD.