Role of genistein on EGFR phosphorylation in acute liver damage induce by administration of carbon tetrachloride in rat (653.17)

Abstract

Our aim was to study the effect of genistein on EGFR phosphorylation on liver rats with acute damage induces by administration of carbon tetrachloride (CCl4). For this study, we used male Wistar rats which were randomly divided into four groups: control, genistein 5mg/kg oral (2 weeks), acute damage CCl4 4mg/kg i.p. (4 weeks) and acute damage+genistein. Rats were sacrificed and liver were collected for determinate EGFR expression and tyrosine phosphorylation by western blot and immunoprecipitation. EGFR expression was elevated in groups with acute liver damage and acute damage+genistein. On the other hand when we analyzed EGFR phosphorylation we founded a reduction on Tyr845 EGFR phosphorylation in group with liver damage, nevertheless the genistein increased this phosphorylation 5 fold compared with CCl4 group, on Tyr1045 the phosphorylation was to decrease in acute damage and acute damage+genistein group. On Tyr992 and Tyr1068 EGFR phosphorylation genistein and acute damage not showed modification. In conclusion, the genistein can be involved in process like proliferation, apoptosis and EGFR degradation in the liver during acute damage, because it modifies specific tyrosine phosphorylation in EGFR.