Abstract
Bioinformatic analysis of snoaL, snoaM, and snoaE genes from Streptomyces nogalater, a microorganism producing the anthracycline antibiotic nogalamycin, is reported. The results of phylogenetic analysis demonstrated high similarity of SnoaL, SnoaM, and SnoaE proteins to cyclases involved in the biosynthesis of various antibiotics. Insertional inactivation of polyketide cyclase genes in the chromosome of S. nogalater Lv65 was performed, and S. nogalater strains MI, LI, and EI were thus obtained. Inactivation of the above named genes did not affect the morphologic characteristics of S. nogalater. The inability of strains MI, LI, and EI to synthesize nogalamycin was demonstrated. Antibiotic synthesis was restored when extra copies of snoaL, snoaM, and snoaE genes were introduced into the above named strains. Streptomycete strains obtained in the present work, as well as nogalamycin cyclase genes, can be used in combinatorial biosynthesis of anthracyclin antibiotics.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
