Abstract
It was reported that human hematopoietic stem cells in bone marrow were restricted to the CD34 +KDR + cell fraction. We found that expression levels of Flk-1, a mouse homologue of KDR, were low or undetectable in mouse Lin −c-Kit +Sca-1 +CD34 low/− cells as well as Hoechst33342 − cells (side population), which have long-term reconstitution capacity. Furthermore, neither Flk-1 +CD34 low/− cells nor Flk-1 +CD34 + cells had long-term reconstitution capacity in mouse. Taken together with other observations using Flk-1-deficient mice, these results indicate that Flk-1 is essential for the development of hematopoietic stem cells in embryo but not for the function of hematopoietic stem cells in adult mouse bone marrow.
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