Role of flagellum and chemotactic motility ofVibrio anguillarumfor phagocytosis by and intracellular survival in fish macrophages

Abstract

The role of the flagellum and chemotactic motility of Vibrio anguillarum for phagocytosis by and intracellular survival in fish macrophages was determined using a wild-type strain, a mutant without the flagellum, a mutant with a truncated flagellum and a non-chemotactic mutant. For all strains, the numbers of intracellular bacteria were relatively low and fell steadily during the observation period. The presence of a flagellum did not influence the uptake by the macrophages, but the smooth swimming phenotype of a non-chemotactic mutant increased its intracellular presence. We suggest that this is due to an increased collision between the mutant and the macrophage, due to a higher average speed of the non-chemotactic mutant.