Year-end Sale % OFF 
Abstract
BackgroundSarcoidosis is a multisystemic granulomatous disease with still unknown etiology. Our previous studies showed a significantly higher percentage of CD34 + cells in the peripheral blood in patients with sarcoidosis (SA) compared to the control group. The objective of the present study was to characterized of the CD34 + cell population in peripheral blood in patients with SA with reference to the control group. Moreover in patients with SA, fibrocytes and endothelial cells were analysed and their relationship to the fibrosis process based on assessment of diffusing capacity for carbon monoxide (DLCO).MethodsData from patients diagnosed with SA at Military Institute of Medicine (Warsaw, Poland) between January 2018 and December 2019 were collected and analysed ongoing basis. Peripheral blood was collected from 26 patients with newly diagnosed pulmonary SA and 16 healthy subjects. The immunomagnetic method and flow cytometry were used. Among the CD34+ progenitor cells were assessed: low-differentiated cells, hematopoietic progenitor cells and endothelial progenitor cells. The Statistica 12.0 software was used for a statistical analysis.ResultsWe observed a significantly higher percentage of low-differentiated cells (13.8 vs. 2.3, P = 0.001) and endothelial cells (0.3 vs. 0.0, P = 0.001) in patients with SA compared to the control group. In the study group the median proportion of fibrocytes was 1.877% (0.983–2.340) in patients with DLCO< 80%, while in patients with DLCO> 80% was 0.795% (0.139–1.951) (P = 0.72). The median proportion of endothelial progenitor cells was higher in patients with DLCO< 80%: 0.889% (0.391–1.741), than in patients with DLCO> 80%: 0.451% (0.177–0.857) (P = 0.44).ConclusionsIn conclusion we demonstrated for the first time the immunophenotype of peripheral CD34 + cells with the degree of their differentiation. The study confirmed the involvement of low differentiated cells and endothelial cells in patients with SA.
Highlights
Sarcoidosis is a multisystemic granulomatous disease with still unknown etiology
Recent data obtained from studies assessing the role of fibrocytes in diseases occurring with fibrosis of the lung parenchyma suggest that there is a relationship between the severity of the fibrosis process and the presence of increased number of fibrocytes in peripheral blood (PB) [17]
We observed a significantly higher percentage of lowdifferentiated cells (13.8 vs. 2.3, P = 0.001) in patients with sarcoidosis compared to the control group (Fig. 2)
Summary
Our previous studies showed a significantly higher percentage of CD34 + cells in the peripheral blood in patients with sarcoidosis (SA) compared to the control group. Our previous studies showed a significantly higher percentage of CD34 + progenitor cells in the PB in patients with newly diagnosed sarcoidosis compared to the control group and showed a positive correlation with CD4/CD8 ratios. Granulomatous inflammation can develop into pulmonary fibrosis - this occurs in about 20% of patients with sarcoidosis [14, 15]. The fibrocytes leaving the bone marrow become circulating mesenchymal progenitor cells associated with several fibrotic disorders, such as pulmonary fibrosis. They are the precursors of fibroblasts that produce tissue proteins in the form of collagen. No literature characterizing the population of CD34+ cells in patients with sarcoidosis has been found
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
