Abstract
We have recently realized megakaryocyte (MK) colony formation in culture from blood and bone marrow progenitors using the plasma clot technique. In this study, the MK stimulating factor was an erythropoietin (Epo) either a poorly purified one(step III from anaemic sheep serum, a crude serum from anaemic mice, an urinary human Epo) or a highly purified one (GOLDWASSER). Similar results were obtained with all these Epo. A linear relationship was found between the number of colonies and seeded cells. However with less than 5.105 plated cells from the blood, no MK colonies were obtained, although erythroid colonies could be grown. In contrast, without Epo, spontaneous colonies could be observed which represented 1/5 th of the maximum plating efficiency , in these eases no erythroid colonies were present. These data suggest that Epo itself acts an a MK colony stimulating factor; but is not the only factor involved in the formation of MK colonies. This in vitro technique will be useful of in determining the factors regulating megakaryocytopoiesis.
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