Abstract
The particulate material was fractionated into 5 size classes (>90μ, 50–90μ, 6–50μ, 1–6μ, and <1μ). DOP was analysed as easily (DOPh, DOPpa) and less easily hydrolysable compounds (DOPox). Based on Vmax, 94% of the high affinity AP activity was due to <50μ cells and 77% to <1μ cells. 83% of the low affinity activity was due to >90μ cells. The high affinity activities were negatively correlated with DOP for the <50μ classes. These correlations came mostly from DOPox. They were more significant when NO3+NO2 concentrations were high, when DIP concentrations were low and when N/P ratio was >10. At lower N/P ratios, AP was more significantly correlated with DIP. The low affinity activities showed significant negative correlation with DIP and with DOP and DOPox for the >90μ class. The inhibition of AP activities by DOPox may originate from stable compounds interfering with DIP for the control of AP synthesis.
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