Abstract
Abstract : The purpose of the above titled postdoctoral trainee ship was to complete several tasks focused upon further establishing the role of EST in the bioactivation of the cooked-food mutagen and breast carcinogen N-OH- PhIP and establishing how bioflavonoids may be used to prevent this bioactivation process. Task #1, as outlined in the statement of work, listed several goals to help define the involvement of EST in bioactivation of N-OH-PhIP in the normal human breast cell and tissue. Part of this task, characterizing the expression and localization of EST in normal human breast cells and tissue, has opened many new research opportunities. Although EST had previously been detected in cultured human mammary epithelial cells, EST had never been detected or its localization determined in normal human breast tissue. However, by using immunohistochemistry we have for the first time localized EST to the epithelial cells lining the ducts and alveoli of normal human breast tissue. This finding is not only novel but is interesting in that these very ductal epithelial cells are believed to be the site for tumor initiation by various carcinogens, including the breast carcinogen N-OH-PhIP.
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