Abstract
Role of deoxycytidylate deaminase in deoxyribonucleotide synthesis in bacteriophage T4 DNA replication
Highlights
simultaneously to two cultures infected by T4D
Cultures infected by cd phage caused about a
VHluridine over that shown by T4D infection
Summary
These deoxyribonucleotides are synthesized at a constant ratio of 2.1 to 1, respectively, exactly reflecting the ratio of thymine to 5-hydroxymethylcytosine in T4 DNA This ratio of synthesis is maintained even on infection by T4 Dnamutants until the deoxyribonucleotides reach very high concentrations [2]. Hall and Tessman in 1966 described phage T4 mutants (cd) unable to induce the formation of dCMP deaminase and considered that the enzyme could have an accessory role in the synthesis of dTMP [12]. Our experiments with T4 Dna- mutants appear to rule against a feedback-sensitive regulatory role for the enzyme in the in vivo control of the synthesis of dTMP and HmdCMP [2]. The present studies show that this enzyme has an important quantitative role in augmenting the synthesis of dTMP and in maintaining the 2:l Thy/HmCyt ratio
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