Role of CYP51 in the Regulation of T3 and FSH-Induced Steroidogenesis in Female Mice.

Abstract

Cytochrome P450 lanosterol 14α-demethylase (CYP51) is a key enzyme in sterol and steroid biosynthesis that is involved in folliculogenesis and oocyte maturation, which is regulated by follicle-stimulating hormone (FSH), as a key reproductive hormone during follicular development. Thyroid hormone (TH) is also important for normal reproductive function. Although 3,5,3'-triiodothyronine (T3) enhances FSH-induced preantral follicle growth, whether and how TH combines with FSH to regulate CYP51 expression during the preantral to early antral transition stage is unclear. The objective of this study was to determine the cellular and molecular mechanisms by which T3 and FSH regulate CYP51 expression and steroid biosynthesis during preantral follicle growth. Our results indicated that CYP51 expression was upregulated in granulosa cells by FSH, and this response was enhanced by T3. Moreover, knockdown CYP51 decreased cell viability. Meanwhile, gene knockdown also blocked T3 and FSH-induced estradiol (E2) and progesterone (P4) synthesis. These changes were accompanied by upregulation of phospho-GATA-4 content. Results of small interfering RNA analysis showed that knockdown of GATA-4 significantly diminished CYP51 gene expression as well as E2/P4 levels. Furthermore, thyroid hormone receptor β was necessary to the activation of phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt), which was required for the regulation of CYP51 expression; activated GATA-4 was also involved these processes. Our data demonstrate that T3 and FSH cotreatment potentiates cellular development and steroid biosynthesis via CYP51 upregulation, which is mediated through the activation of the PI3K/Akt pathway. Meanwhile, activated GATA-4 is also involved in this regulatory system. These findings suggest that CYP51 is a mediator of T3 and FSH-induced follicular development.