Role of CSE1034 in bacterial lipids and polysaccharides involved in biofilm formation: a comparison with other drugs

Abstract

In the present investigation minimum inhibitory concentration (MIC) and minimum biofilm eradication concentration (MBEC) and CSE1034 (Sulbactomax) was compared with piperacilline+tazobactam, amoxyclave, ceftriaxone, ceftriaxone+sulbactam and cefoperazone+ sulbactam in planktonic and sessile cells of Acinetobacter baumannii, Enterobacter cloacae and Pseudomonas aeruginosa. MICs were determined by broth microdilution method with a final inoculum size of 106 cfu/ml of plaktonic cells. MBECs were measured using a calgary biofilm device method to establish a co-relation with biofilm breaking efficacy of different drugs. The MICs for CSE1034 ranged from 0.5 to 4.0 μg/ml and for other antibacterial drugs ranged from 2 to 32 μg/ml. The MBEC for CSE1034 ranged from 8 to 16 μg/ml and for other antibacterial agents, it ranged from 64 to 4096 μg/ml. The CSE1034 exhibited approximately 5 logs reduction in the number of bacteria present in biofilm when compared with other antibacterial agents. When total lipid and total polysaccharide contents were compared, CSE1034 showed 90 and 84% reduction, respectively. The enhanced efficacy of CSE1034 in the eradication of biofilm infection is due to presence of EDTA which helps in the destabilizing of the barriers responsible for the development of biofilm as well as antibiotic resistance. In conclusion, combining of ceftriaxone+sulbactam with EDTA can significantly reduces the MIC and MBEC values against selected organisms. Hence, CSE1034 could be one of the best choices to eradicate the biofilm caused by these organisms. Key words: Lipopolysaccharide, lipid, log reduction, antibacterial agents.