Role of cotton sticks biochar in immobilization of nickel under induced toxicity condition and growth indices of Trigonella corniculata L.

Abstract

Among various heavy metals, nickel (Ni) is a potential pollutant that accumulates in broad-leaf vegetables and is reported to be carcinogenic. Biochar (BC) is a nutrient-rich and effective organic amendment for immobilization of Ni in soil. Fenugreek (Trigonella corniculata L.), a broad-leaf vegetable, is commonly cultivated due to its all-inclusive composition of nutrients such as calcium and iron and β-carotene and vitamins. Therefore, a field-pot study was conducted to examine the effectiveness of cotton-sticks-waste biochar (BC) for soil immobilization of Ni in fenugreek crop cultivated between early-October to end-November 2015. Fenugreek was grown in a sandy-loam soil experimentally contaminated with various Ni levels (0, 25, 50, and 100mg Ni kg-1 soil) under three BC levels (0, 3, and 5%; w/w). Overall, results showed increasing plant lipid peroxidation (assessed via malondialdehyde) and ascorbic-acid concentration with increasing Ni toxicity level without BC application (p ≤ 0.05). Application of 3% BC increased the chlorophyll a (20.0%), chlorophyll b (49.1%), total chlorophyll (27.6%), carotenoids (21.6%), anthocyanin (27.2%), photosynthetic rate (112%), transpiration rate (45.0%), and sub-stomatal CO2 concentration (19.9%) in fenugreek as compared to control (0% BC) under 50mg Ni kg-1 soil. Higher BC application rate (5%) was more effective in increasing the chlorophyll a (33.6%), chlorophyll b (81.1%), total chlorophyll (43.9%), carotenoids (71.7%), anthocyanin (77.8%), photosynthetic rate (127%), transpiration rate (42.2%), and sub-stomatal CO2 concentration (23.5) over control under 100mg Ni kg-1 soil. We suggest that the consistent increases in dry mass, carbon flux rate and, protein, amino acids, and sugar contents of fenugreek (cultivated in a soil toxified with Ni and amended with 5% BC) seems to be caused by the reduction in the mobility of Ni in the presence of BC in a sandy-loam soil.