Abstract
The influence of column temperature on chromatographic performance was examined for reversed-phase, normal-phase and cation-exchange columns in open-tubular microcapillary liquid chromatography. Linear relationships between the logarithm of capacity factor and the reciprocal of column temperature were generally observed. Operation at higher column temperature had the tendency to give higher column efficiency owing to the decrease in the viscosity of the mobile phase.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have