Role of Cold Climate and Freeze–Thaw on the Survival, Transport, and Virulence of Yersinia enterocolitica

Abstract

Surface and near-surface soils in cold climate regions experience low temperature and freeze-thaw (FT) conditions in the winter. Microorganisms that are of concern to groundwater quality may have the potential to survive low temperature and FT in the soil and aqueous environments. Although there is a body of literature on the survival of pathogenic bacteria at different environmental conditions, little is known about their transport behavior in aquatic environments at low temperatures and after FT. Herein, we studied the survival, transport, and virulence of a Gram-negative bacterial pathogen, Yersinia enterocolitica, when subjected to low temperature and several FT cycles at two solution ionic strengths (10 and 100 mM) in the absence of nutrients. Our findings demonstrate that this bacterium exhibited higher retention on sand after exposure to FT. Increasing the number of FT cycles resulted in higher bacterial cell surface hydrophobicity and impaired the swimming motility and viability of the bacterium. Moreover, the transcription of flhD and fliA, the flagellin-encoding genes, and lpxR, the lipid A 3'-O-deacylase gene, was reduced in low temperature and after FT treatment while the transcription of virulence factors such as ystA, responsible for enterotoxin production, ail, attachment invasion locus gene, and rfbC, O-antigen gene, was increased. Y. enterocolitica tends to persist in soil for long periods and may become more virulent at low temperature in higher ionic strength waters in cold regions.