Abstract
Abstract In vitro chromium(VI) is unreactive toward DNA under physiological conditions. Therefore, the ability of chromium(VI) to damage DNA depends on the presence of cellular components capable of forming “reactive intermediates”; upon reaction with chromium(VI). We have examined the role of glutathione and hydrogen peroxide in chromium(VI)‐induced DNA damage in vitro. Reaction of glutathione with chromium(VI) produced significant levels of two chromium(V) complexes and glutathione thiyl radical, whereas reaction of chromium(VI) with hydrogen peroxide produced hydroxyl radical without producing detectable levels of chromium(V). Reaction of DNA with chromium(VI) in the presence of glutathione resulted in Cr‐DNA adducts with little DNA strand breakage. Reaction of DNA with chromium(Vl) in the presence of hydrogen peroxide produced the 8‐hydroxydeoxyguanosine adduct and extensive DNA strand breakage in the absence of significant Cr‐DNA adduct formation. These results suggest that the nature of chromium(VI)...
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