Role of cholinergic-muscarinic receptors in visual discrimination performance of rats: Importance of stimulus load

Abstract

Central cholinergic transmission has long been implicated in various cognitive processes, including memory acquisition, consolidation, and attentional processes. Here, we examined the role of muscarinic receptors in visual discrimination performance under conditions of altered visual information availability. Adult rats were trained to discriminate two visual cues (indicating the presence and absence of a hidden escape platform, respectively) in a water maze-based, trapezoidal-shaped apparatus. Following task acquisition, testing continued with two types of trials: regular trials (RTs; both visual cues present, identical to training conditions) and probe trials (PTs; only one of the two cues present). In Experiment 1, removal of one visual cue on PTs impaired discrimination performance. Moreover, scopolamine administration (0.125–1.0mg/kg, i.p.) tended to further suppress performance in a dose-dependent manner on PTs, while discriminations on RTs were left intact. In Experiment 2, these results were confirmed and extended by showing that PT (one visual cue) performance could improve with training in undrugged, but not in scopolamine-treated rats. Together, these experiments reveal that visual discrimination performance of rats benefits from the concurrent availability of two visual cues that provide complimentary and consistent information. Furthermore, muscarinic receptors are particularly important under conditions of reduced visual information availability, as well as in the adoption of new behavioral strategies, such as switching from two-cue to single-cue guided navigation.