Abstract
Purpose: The aim of the current study was to assess the prognostic value of theChk1 gene in theDNA damage responsepathway in gastric cancer (GC). Methods: Expression levels of the Chk1 were measured in 220 GC tumor tissues and adjacent healthy/noncancerous tissues using real-time PCR and immunohistochemical staining. Genomic instability in GC patients was measured using the long-run real-time PCR technique for DNA-damage quantificationassay and comet assay. Results: Significantly downregulated expression of Chk1 was observed at themRNA level (p<0.0001) and protein level (p<0.0001). Significantly increased frequency of lesions/10kb and comets was observed in tumor tissues compared with control tissues. Conclusion: The data suggest that downregulated expression of Chk1and positiveHeliobacter pyloriinfection status may haveprognostic significance in GC.
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