Role of CD200 and CD43 in Diagnosis and Prognosis of CLL and NHL Patients

Abstract

Background: Chronic lymphoproliferative disorder (LPD), is a malignant disease of lymphocytes in the blood and lymphatic tissue. Chronic lymphocytic leukaemia, it is the commonest type of chronic lymphoproliferative disorder. Scoring by immunophenotyping is used to differentiate B-cell chronic lymphocytic leukemia from other B-Non-Hodgkin lymphomas. CD200 (OX2) is a glycoprotein of membrane, it is related to type I superfamily of immunoglobulin . CD43 (Sialophorin) is a sialoglycoprotein that is present on the surface of T lymphocytes, some B lymphocytes, granulocytes and monocytes, that play important role for immune function and also play a role as physiologic ligand-receptor complex involved in T- cell activation. Aim of study-Evaluate the role of CD200 and CD43 positive expression and co-expression in diagnosis and prognosis of CLL and NHL. Subjects and Methods: This cross sectional study on one hundred forty five patients with chronic lymphoprliferative disorders who were attending Baghdad teaching hospital at medical city from beginning of January 2020 to end of December 2020, patients divided in to two groups; chronic lymphocytic leukemia (CLL) and non-Hodgkin lymphoma (NHL) patients At diagnosis, there was assessment of CD200 and CD43. Clinical and laboratory data were done including staging by modified Rai, and Ann arbor staging system ( for CLL and NHL respectively), then follow up for about 6-12. Results: There is significant statistical correlation between CD 200 and CD 43 and often co-expression of both in differentiation between CLL and NHL (p value < 0.001). Almost all patients of CD 200 positive expression show moderate to bright pattern of expression in CLL in apposite to NHL patients, who showed dim to moderate pattern of expression, while the great majority of CD43 expression was dim to moderate pattern in both CLL and NHL patients.In CLL patients there was no significant correlation between prognostic markers (age, Hb, platelets count, lymphocytes count and CD 38 expression) and CD200, CD 43. For NHL patients, all markers show no significant correlation except that CD 43 show significant correlation with expression of CD 38. Conclusion: CD200, CD43 and often co-expression of both have a significant value in diagnosis and differentiation of CLL from B- NHLs.